Search for Mu insertions near your gene of interest
This interface provides access to a subset of the Mu insertions detected by Mu-Illumina (PubMed) sequencing during mutant cloning efforts involving the Photosynthesis Mutant Library (PML). The posted insertions map between 150 bp upstream of the annotated start codon and 150 bp downstream of the annotated stop codon of gene models in the Filtered Gene Set from Maize Genome Assembly AGPv3 (www.gramene.org). Insertions that map more distant to genes rarely disrupt gene expression; due to limited resources, we are not making these available.
Our approach ensures that the vast majority of insertions posted here are heritable. However, on rare occasions, insertions are not found in the siblings of the plant that was sacrificed for DNA extraction. If you are unable to amplify the insertion from the siblings we provide, please let us know. We can help you determine whether this is a technical problem or whether the insertion is missing from siblings of the sequenced individual.
We have a large volume of requests and our seed stocks are becoming depleted. Therefore, we generally provide seed only to laboratories in the United States.
This resource currently includes insertions near/within loci.
This resource is made possible through funding from National Science Foundation grant IOS-1339130: Translational Dynamics of Leaf and Chloroplast Development in Maize.